|
KMID : 0545120150250030381
|
|
Journal of Microbiology and Biotechnology
2015 Volume.25 No. 3 p.381 ~ p.385
|
|
|
Viable Bacterial Cell Patterning Using a Pulsed Jet Electrospray System
|
|
Chong Eui-seok
Hwang Gi-Byung
Kim Kyoung-Tae
Lee Im-Soon
Han Song-Hee
Kim Hyung-Joo
Kim Sung-Jin
Jung Hyo-Il
Lee Byung-Uk
|
|
|
Abstract
|
|
|
|
In the present study, drop-on-demand two-dimensional patterning of unstained and stained bacterial cells on untreated clean wafers was newly conducted using an electrospray pulsed jet. We produced various spotted patterns of the cells on a silicon wafer by varying the experimental conditions, such as the frequency, flow rate, and translational speed of the electrospray system in a two-dimensional manner. Specifically, the electrospray’s pulsed jet of cell solutions produced alphabetical patterns consisting of spots with a diameter of approximately 10 μm, each of which contained a single or a small number of viable bacteria. We tested the viability of the patterned cells using two visualization methods. This pattering technique is newly tested here and it has the potential to be applied in a variety of cell biology experiments.
|
|
|
KEYWORD
|
|
|
electrospray, two-dimensional pattern, bacteria treatment methods, pulsed jet, microorganism arrays, bioaerosols
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|